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SibEnzyme Ltd. :: Display product information: Rsa I
Name
Rsa I
Catalog numberE113E114
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteGT^AC
CA^TG
Methylation sensitivitynot tested
OriginRhodopseudomonas sphaeroides
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA, 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10050 - 750 - 1050 - 7575 - 100

References: Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F. J.Bacteriol. 142: 380-383 (1980).