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SibEnzyme Ltd. :: Display product information: Pvu II
Pvu II
Catalog numberE111E112
Package, u.a.200010000
Concentration, u.a./ml1000010000

Recognition siteCAG^CTG
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Pvu II gene from Proteus vulgaris
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
25 - 5010025 - 5025 - 5025 - 50

Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Repin, V.E., Degtyarev, S.Kh., Unpublished observations.