|Catalog number||E111||E112 |
|Package, u.a.||2000||10000 |
|Concentration, u.a./ml||10000||10000 |
|Methylation sensitivity||not tested|
|Origin||E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||80oC|
|Optimum SE-buffer||G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA|
Enzyme % activity:
|25 - 50||100||25 - 50||25 - 50||25 - 50|
Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Repin, V.E., Degtyarev, S.Kh., Unpublished observations.