|Catalog number||E255||E256 |
|Package, u.a.||100||500 |
|Concentration, u.a./ml||2000-5000||2000-5000 |
|Methylation sensitivity||Blocked by overlapping Dcm methylation(CmCWGG): RGGWCCTGG.|
|Origin||Pseudomonas species PP|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C. Store at -70ºC is recommended for period longer than 30 days.|
|Assayed on||Lambda DNA (dcm-,HindIII-digest)|
|Ligation||After 5-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and more than 80% of these can be recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)|
Enzyme % activity:
|10 - 25||10 - 25||0 - 10||0 - 10||100|
Note: To obtain 100% activity, BSA should be added
to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (1996).