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SibEnzyme Ltd. :: Display product information: PspPP I
Catalog numberE255E256
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition siteRG^GWCCY
Methylation sensitivityBlocked by overlapping Dcm methylation(CmCWGG): RGGWCCTGG.
OriginPseudomonas species PP
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C. Store at -70ºC is recommended for period longer than 30 days.
Assayed onLambda DNA (dcm-,HindIII-digest)
LigationAfter 5-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and more than 80% of these can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
10 - 2510 - 250 - 100 - 10100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (1996).