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SibEnzyme Ltd. :: Display product information: PspOM I
Name
PspOM I
Catalog numberE215E216
Package, u.a.10005000
Concentration, u.a./ml1000010000

Recognition siteG^GGCCC
CCCGG^G
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164
Storage conditions20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 ug/ml BSA; and 50% glycerol. Store at -20C.
Assayed onLambda DNA (BamHI-digest)
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10010 - 250 - 100 - 10100

Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,13.
References: Belichenko, O.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1994).