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SibEnzyme Ltd. :: Display product information: DraIII
Name
DraIII
Catalog numberE309E310
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteCACNNN^GTG
GTG^NNNCAC
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus
Storage conditions10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-buffer2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
25 - 5050 - 7575 - 10075 - 10050 - 75

Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: de Wit, C.M., Dekker, B.M.M., Neele, A.C., de Waard, A., FEBS Lett. 180: 219-223 (1985) Grosskopf, R., Wolf, W., Kessler, C., Nucleic Acid res. 13: 1517-1528 (1985)