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Name PspN4 I Catalog number E089 E090 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-30000 10000-30000 Recognition site GGN^NCC CCN^NGG Methylation sensitivity Blocked by 5`-GGNN(5mC)C-3`/3-C(5mC)NNGG-5` or 5`-GGNN(5mC)C-3`/3-CCNNGG-5` methylation Origin Pseudomonas species N4 Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After 10-fold overdigestion with enzyme more than 950% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme % activity: B G O W Y 10 - 25 10 - 25 10 - 25 25 - 50 100 References: Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (1995).