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SibEnzyme Ltd. :: Display product information: PspN4 I
Name
PspN4 I
Catalog numberE089E090
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteGGN^NCC
CCN^NGG
Methylation sensitivityBlocked by
5`-GGNN(5mC)C-3`/3-C(5mC)NNGG-5` or
5`-GGNN(5mC)C-3`/3-CCNNGG-5` methylation
OriginPseudomonas species N4
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 950% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2510 - 2510 - 2525 - 50100

References: Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (1995).