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Name PspE I Catalog number E169 E170 Package, u.a. 2000 10000 Concentration, u.a./ml 5000-10000 5000-10000 Recognition site G^GTNACC CCANTG^G Methylation sensitivity not tested Origin Pseudomonas species E Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Enzyme % activity: B G O W Y 100 50 - 75 25 - 50 50 - 75 50 - 75 Note: High enzyme concentration may result in star activity. References: Dedkov, V.S., Rechkunova, N.I., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994).