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SibEnzyme Ltd. :: Display product information: Psp124B I
Psp124B I
Catalog numberE107E108
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteGAGCT^C
Methylation sensitivitynot tested
OriginPseudomonas species 124B
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
75 - 10010010 - 250 - 1075 - 100

References: Degtyarev, S.Kh., Dedkov, V.S., Rechkunova, N.I., Morgan, R. Unpublished observations (1994).