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SibEnzyme Ltd. :: Display product information: Psi I
Name
Psi I
Catalog numberE279E280
Package, u.a.100500
Concentration, u.a./ml5000-100005000-10000

Recognition siteTTA^TAA
AAT^ATT
Methylation sensitivitynot tested
OriginPseudomonas species-SE-G49
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10025 - 5010 - 2525 - 5075 - 100

Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,25.

References:

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