|Catalog number||E463||E464 |
|Package, u.a.||200||1000 |
|Concentration, u.a./ml||10000||10000 |
|Methylation sensitivity||not tested|
|Origin||E.coli strain that carries the cloned Zra I gene from Zoogloea ramigera 11|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20*C.|
|Assayed on||Lambda DNA|
|Ligation||After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||80oC|
|Optimum SE-buffer||B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)|
Enzyme % activity:
|100||50 - 75||25 - 50||25 - 50||75 - 100|
Note: High enzyme concentration may result in star activity.
The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5.
ZraI cleaves linear plasmid DNA at a rate 1,5-2 times higher than supercoiled plasmid DNA.
References: Dedkov, V.S., Sinichkina, S.A., Popichenko, D.V., Degtyarev, S.K.
Biotekhnologia 6 : 3-7 (2001)