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SibEnzyme Ltd. :: Display product information: ZraI
Name
ZraI
Catalog numberE463E464
Package, u.a.2001000
Concentration, u.a./ml1000010000

Recognition siteGAC^GTC
CTG^CAG
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned Zra I gene from Zoogloea ramigera 11
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20*C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.)

Enzyme % activity:
BGOWY
10050 - 7525 - 5025 - 5075 - 100

Note: High enzyme concentration may result in star activity. The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5. ZraI cleaves linear plasmid DNA at a rate 1,5-2 times higher than supercoiled plasmid DNA.
References: Dedkov, V.S., Sinichkina, S.A., Popichenko, D.V., Degtyarev, S.K. Biotekhnologia 6 : 3-7 (2001)