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SibEnzyme Ltd. :: Display product information: Ple19 I
Name
Ple19 I
Catalog numberE195E196
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition siteCGAT^CG
GC^TAGC
Methylation sensitivityNot blocked by overlapping Dam-methylation (GmATC): CGATCG
OriginPseudomonas lemoignei 19
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10075 - 10025 - 5025 - 50100

References: Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).