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SibEnzyme Ltd. :: Display product information: Pci I
Pci I
Catalog numberE275E276
Package, u.a.2001000
Concentration, u.a./ml1000010000

Recognition siteA^CATGT
Methylation sensitivityNot blocked by ACmATGT methylation.
Blocked by mACATGT methylation.
OriginE.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; and 50% glycerol; Store at -20C.
Assayed onT7 DNA
LigationAfter 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of T7 DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
50 - 7575 - 10010075 - 10050 - 75

Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,13.
References: Abdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1998).