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SibEnzyme Ltd. :: Display product information: Ags I
Ags I
Catalog numberE573E574
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteTTS^AA
Methylation sensitivitynot tested
OriginAgrococcus species 25
Storage conditions10 mM Tris-HCl(pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
75 - 10050 - 7510 - 2510 - 25100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.