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Name Ags I Catalog number E573 E574 Package, u.a. 200 1000 Concentration, u.a./ml 5000 5000 Recognition site TTS^AA AA^STT Methylation sensitivity not tested Origin Agrococcus species 25 Storage conditions 10 mM Tris-HCl(pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA Enzyme % activity: B G O W Y 75 - 100 50 - 75 10 - 25 10 - 25 100 Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml. Do not use BSA for long incubation.