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SibEnzyme Ltd. :: Display product information: Ars I
Name
Ars I
Catalog numberE575E576
Package, u.a.50250
Concentration, u.a./ml500500

Recognition site^(N)8GACNNNNNNTTYG(N)11^
^(N)13CTGNNNNNNAARC(N)6^
Methylation sensitivitynot tested
OriginArthrobacter species NTS
Storage conditions10 mM KPO4(pH 7.4); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20C.
Assayed onT7 DNA
LigationAfter 3-fold overdigestion with enzyme 70% of DNA fragments can be ligated. Of these 80% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 1 u.a. of enzyme for 16 hours at 30C.
Optimum temperature30 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
0 - 100 - 100 - 100 - 10100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.