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Name Gsa I Catalog number E563 E564 Package, u.a. 1000 5000 Concentration, u.a./ml 20000 20000 Recognition site CCCAG^C G^GGTCG Methylation sensitivity not tested Origin Geobacillus stearothermophilus Y Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of DNA with 20 u.a. of enzyme for 16 hours at 70°C. Optimum temperature 70 oC Inactivation 20 minutes No Optimum SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA Enzyme % activity: B G O W Y 10 - 25 25 - 50 75 - 100 100 75 - 100 Note: Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.