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SibEnzyme Ltd. :: Display product information: Gsa I
Gsa I
Catalog numberE563E564
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition siteCCCAG^C
Methylation sensitivitynot tested
OriginGeobacillus stearothermophilus Y
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 20 u.a. of enzyme for 16 hours at 70C.
Optimum temperature70 oC
Inactivation 20 minutesNo
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
10 - 2525 - 5075 - 10010075 - 100

Note: Do not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.