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SibEnzyme Ltd. :: Display product information: BstAF I
Name
BstAF I
Catalog numberE135E136
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition siteC^TTAAG
GAATT^C
Methylation sensitivitynot tested
OriginBacillus stearothermophilus AF
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 40% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
10 - 2525 - 5075 - 10010025 - 50

Note: Do not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.