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SibEnzyme Ltd. :: Display product information: Dri I
Dri I
Catalog numberE193E194
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition siteGACNNN^NNGTC
Methylation sensitivitynot tested
OriginDeinococcus radiodurans EA
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated. Ligation is better in presence of 10% PEG.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
75 - 10075 - 10010 - 2510 - 25100

References: Abdurashitov, M.A., Nayakshina, T.N., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2003)