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SibEnzyme Ltd. :: Display product information: Acl I
Acl I
Catalog numberE011E012
Package, u.a.2001000
Concentration, u.a./ml3000-50003000-5000

Recognition siteAA^CGTT
Methylation sensitivityBlocked by CG methylation
OriginAcinetobacter calcoaceticus
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 0.05% Triton X-100; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
0 - 100 - 100 - 100 - 10100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Degtyarev, S.Kh., Abdurashitov, M.A., Kolyhalov, A.A., Rechkunova, N.I., Nucleic Acids Res. 20: 3787 (1992).