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Name Acl I Catalog number E011 E012 Package, u.a. 200 1000 Concentration, u.a./ml 3000-5000 3000-5000 Recognition site AA^CGTT TTGC^AA Methylation sensitivity Blocked by CG methylation Origin Acinetobacter calcoaceticus Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 0.05% Triton X-100; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA Enzyme % activity: B G O W Y 0 - 10 0 - 10 0 - 10 0 - 10 100 Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml. Do not use BSA for long incubation. References: Degtyarev, S.Kh., Abdurashitov, M.A., Kolyhalov, A.A., Rechkunova, N.I., Nucleic Acids Res. 20: 3787 (1992).