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SibEnzyme Ltd. :: Display product information: Aat II
Aat II
Catalog numberE287E288
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteGACGT^C
Methylation sensitivitynot tested
OriginE.coli strain, that carries the cloned Aat II gene from Acetobacter aceti
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -200C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
10 - 2525 - 5010 - 2525 - 50100

Note: High enzyme concentration may result in star activity.
References: Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Nucleic Acid Res. 10:5747-5752(1982)