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Additional information about degenerate nucleotides and the icons description - in the legeng at page bottom.

 
Search for restriction endonuclease
Enzyme's name (withous spaces)
Recognition sequence (including A, G, T, C, mA, mC and degenerate nucleotides)
Part of enzyme's recognition sequence (A, G, T, C, mA and mC)
 
EnzymePrototypeRecognition SequenceCat #Package, u.a.Price, euroNoteOrder
Aat IIAat II
GACGT^C
C^TGCAG
E28750035
  
E2882500140
Abs IAbs I
CC^TCGAGG
GGAGCT^CC
E5355050
    
E536250200
Acc16 IMst I
TGC^GCA
ACG^CGT
E00120015
  
E002100060
Acc36 IBspM I
ACCTGC(N)4^
TGGACG(N)8^
E28910025
  
E290500100
Acc65 IKpn I^
G^GTACC
CCATG^G
E003100020
  Blocked by GGTAC(5mC) methylation  
E004500080
AccB1 IHgiC I
G^GYRCC
CCRYG^G
E1635005
  
E164250020
AccB7 IPflM I
CCANNNN^NTGG
GGTN^NNNNACC
E17920010
  Blocked by CCANNNN(5mc)TGG or 
C(5mC)ANNNNNTGG methylation   
E180100040
AccBS IBsrB I
GAG^CGG
CTC^GCC
E007100040
    
E0085000160
Acl IAcl I
AA^CGTT
TTGC^AA
E01120025
  Blocked by 
AA(5mC)GTT methylation   
E0121000100
AclW IBin I
GGATC(N)4^
CCTAG(N)5^
E21110030
  Blocked by 
 GG(6mA)TC methylation   
E212500120
Aco ICfr I
Y^GGCCR
RCCGG^Y
E49910025
  Blocked by 
(5mc)YGGCCR methylation   
E500500100
Acs IApo I
R^AATTY
YTTAA^R
E01350030
  
E0142500120
Acu IEco57 I
CTGAAG(N)16^
GACTTC(N)14^
E451508
  
E45225032
Afe IEco47 III
AGC^GCT
TCG^CGA
E21320065
  
E2141000260
E214X1000260
Ags IAgs I
TTS^AA
AA^STT
E57320050
  New product  
E5741000200
Ahl ISpe I
A^CTAGT
TGATC^A
E173100075
  
E1745000300
Ajn IEcoR II
^CCWGG
GGWCC^
E47320045
    Not blocked by 
C(5mC)WGG methylation   
E4741000180
Alu IAlu I
AG^CT
TC^GA
E01520020
    
E016100080
AluB IAluB I
AG^CT
TC^GA
E54920015
    
E550100060
Ama87 IAva I
C^YCGRG
GRGCY^C
E017100025
  
E0185000100
Apa IApa I
GGGCC^C
C^CCGGG
E019100010
  Blocked by   
 GGGCC(5mC) methylation  
E020500040
Ars IArs I
^(N)8GACNNNNNNTTYG(N)11^
^(N)13CTGNNNNNNAARC(N)6^
E5755050
  
E576250200
AsiG IAge I
A^CCGGT
TGGCC^A
E23510025
  
E236500100
AspA2 IAvr II
C^CTAGG
GGATC^C
E24550040
    
E2462500160
AspLE IHha I
GCG^C
C^GCG
E22150010
  Blocked by 
5`-G(5mC)GC-3`/3-CG(5mC)G-5` or 
5`-G(5mC)GC-3`/3`-CGCG-5`
methylation   Not blocked by 
5`-GCG(5mC)-3`/3`-(5mC)GCG-5` or 
5`-GCG(5mC)-3`/3`-CGCG-5`
methylation   
E222250040
AspS9 ISau96 I
G^GNCC
CCNG^G
E117100020
  Blocked by 
GGNC(5mC) methylation    
E118500080
AsuC2 ICau II
CC^SGG
GGS^CC
E257200025
  
E25810000100
AsuHP IHph I
GGTGA(N)8^
CCACT(N)7^
E23120025
  Blocked by 
 GGTG(6mA) methylation    
E2321000100
AsuNH INhe I
G^CTAGC
CGATC^G
E06350030
  
E0642500120
BamH IBamH I
G^GATCC
CCTAG^G
E021400015
  Not blocked by 
 GG(6mA)TCC methylation   
E0222000060
E021X400015
E022X2000060
Bar IBar I
^(N)7GAAGNNNNNNTAC(N)12^
^(N)12CTTCNNNNNNATG(N)7^
E54710050
  
E548500200
Bbv12 IHgiA I
GWGCW^C
C^WCGWG
E02320010
  
E024100040
Bgl IBgl I
GCCNNNN^NGGC
CGGN^NNNNCCG
E025100020
  Not blocked by 
GC(5mC)NNNNNGGC methylation   
E026500080
Bgl IIBgl II
A^GATCT
TCTAG^A
E027100025
  Not blocked by 
 AG(6mA)TCT methylation  
E0285000100
Bis IBis I
G(5mC)^NGC
CGN^(5mC)G
E4854080
    
E486200320
Bls IBls I
G(5mC)N^GC
(5mC)G^N(5mC)G
E53310080
    New product  
E534500320
Bme18 IAva II
G^GWCC
CCWG^G
E029100020
  
E030500080
Bmt INhe I^
GCTAG^C
C^GATCG
E4571000160
  
E4585000640
Bmu IBfi I
ACTGGG(N)5^
TGACCC(N)4^
E487100100
  
E488500400
BpmIGsuI
CTGGAG(N)16^
GACCTC(N)14^
E4675040
  
E468250160
Bpu10IBpu10I
CC^TNAGC
GGANT^CG
E14920050
    
E1501000200
Bpu14 IAsu II
TT^CGAA
AAGC^TT
E033100012
  
E034500048
Bsa29 ICla I
AT^CGAT
TAGC^TA
E205100030
  Blocked by (6mA)TCG(6mA)T 
  Blocked by 
AT(5mC)GAT methylation  
E2065000120
Bsc4 IBsiY I
CCNNNNN^NNGG
GGNN^NNNNNCC
E21950030
  
E2202500120
Bse1 IBsr I
ACTGGN^
TGAC^CN
E03550015
  
E036250060
Bse118 ICfr10 I
R^CCGGY
YGGCC^R
E03920025
  
E0401000100
Bse21 ISau I
CC^TNAGG
GGANT^CC
E03750020
    
E038250080
Bse3D IBsrD I
GCAATGNN^
CGTTAC^NN
E25320030
    
E2541000120
Bse8 IBsaB I
GATNN^NNATC
CTANN^NNTAG
E147100025
  
E1485000100
BseP IBseP I
G^CGCGC
CGCGC^G
E18120035
  Blocked by 
G(5mC)G(5mC)GC methylation  
E1821000140
BseX3 IXma III
C^GGCCG
GCCGG^C
E26320020
  Blocked by 
(5mC)GGC(5mC)G methylation  
E264100080
BslF IFin I
GGGAC(N)10^
CCCTG(N)14^
E47910040
  
E480500160
Bso31 IEco31 I
GGTCTC(N)1^
CCAGAG(N)5^
E2851006
  Not blocked by
  GGTCT(5mC) methylation  
E28650024
Bsp13 IBspM II
T^CCGGA
AGGCC^T
E18350020
  Blocked by TCCGG(6mA)
 and (6ma)TCCGGA methylation  
E184250080
Bsp1720 IEsp I
GC^TNAGC
CGANT^CG
E18510010
  
E18650040
Bsp19 INco I
C^CATGG
GGTAC^C
E04750025
  
E0482500100
BspAC IAci I
C^CGC
GGC^G
E50120050
  Blocked by 
C(5mC)GC methylation    
E5021000200
BspFN IFnuD II
CG^CG
GC^GC
E55750025
  Blocked by 
(5mC)G(5mC)G methylation  
E5582500100
BssEC ISec I
C^CNNGG
GGNNC^C
E27320025
    
E2741000100
BssNA ISna I
GTA^TAC
CAT^ATG
E261100050
  
E2625000200
BssT1 ISty I
C^CWWGG
GGWWC^C
E20710006
  
E208500024
Bst2B IBsi I
C^TCGTG
GAGCA^C
E04320030
  
E0441000120
Bst2U IBstN I
CC^WGG
GGW^CC
E05110007
  Not blocked by 
C(5mC)WGG methylation    
E052500028
Bst4C ITsp4C I
ACN^GT
TG^NCA
E26520020
  
E266100080
Bst6 IKsp632 I
CTCTTC(N)1^
GAGAAG(N)4^
E23920020
    
E240100080
BstAC IAcy I
GR^CGYC
CYGC^RG
E09350020
  
E094250080
BstAF IAfl II
C^TTAAG
GAATT^C
E135100010
  
E136500040
BstAP IApaB I
GCANNNN^NTGC
CGTN^NNNNACG
E25920050
  
E2601000200
BstAU IBsp1407 I
T^GTACA
ACATG^T
E26750025
  
E2682500100
BstBA IBsaA I
YAC^GTR
RTG^CAY
E23750040
  Blocked by 
YA(5mC)GTR methylation  
E2382500160
BstC8ICac8I
GCN^NGC
CGN^NCG
E30520035
  
E3061000140
BstDE IDde I
C^TNAG
GANT^C
E22750035
    
E2282500140
BstDS IDsa I
C^CRYGG
GGYRC^C
E083100040
  
E0845000160
BstEN IEcoN I
CCTNN^NNNAGG
GGANNN^NNTCC
E10320010
  Not blocked by 
C(5mC)TNNNNNAGG and CCTNNNN(5mc)AGG methylation  
E104100040
BstF5 I(Fok I )
GGATGNN^
CCTAC^NN
E03150020
  
E032250080
BstFN IFnuD II
CG^CG
GC^GC
E28330015
  Blocked by 
(5mC)G(5mC)G methylation    
E284150060
BstH2 IHae II
RGCGC^Y
Y^CGCGR
E17150010
  
E172250040
BstHH IHha I
GCG^C
C^GCG
E143200015
  Blocked by 
5`-G(5mC)GC-3`/3-CG(5mC)G-5` or 
5`-G(5mC)GC-3`/3`-CGCG-5`
methylation   Not blocked by 
5`-GCG(5mC)-3`/3`-(5mC)GCG-5` or 
5`-GCG(5mC)-3`/3`-CGCG-5`
methylation   
E1441000060
BstKTIMboI
GAT^C
C^TAG
E15120050
  Blocked by 
G(6mA)TC methylation  Not blocked by 
GAT(5mC) methylation  
E1521000200
BstMA IBsmA I
GTCTC(N)1^
CAGAG(N)5^
E291200050
    
E29210000200
BstMB IMbo I
^GATC
CTAG^
E11920020
  Blocked by 
 G(6mA)TC methylation  Not blocked by 
GAT(5mC) methylation  
E120100080
BstMC IMcr I
CGRY^CG
GC^YRGC
E07150020
  
E072250080
BstMW IMwo I
GCNNNNN^NNGC
CGNN^NNNNNCG
E45950080
  
E4602500320
BstNS INsp I
RCATG^Y
Y^GTACR
E25120035
  
E2521000140
BstPA IPshA I
GACNN^NNGTC
CTGNN^NNCAG
E299100050
  
E3005000200
BstSCIScrFI
^CCNGG
GGNCC^
E30710025
  Blocked by 
C(5mC)NGG methylation    
E308500100
BstSF ISfe I
C^TRYAG
GAYRT^C
E19720035
  
E1981000140
BstSL IBseS I
GKGCM^C
C^MCGKG
E56120040
  Blocked by 
GKG(5mC)MC methylation  Not blocked by 
GKGCM(5mC) methylation  
E5621000160
BstSN ISnaB I
TAC^GTA
ATG^CAT
E06520020
  
E066100080
BstV1IBbvI
GCAGC(N)8^
CGTCG(N)12^
E30310035
  
E304500140
BstV2 IBbv II
GAAGAC(N)2^
CTTCTG(N)6^
E29720020
    
E298100080
BstX IBstX I
CCANNNNN^NTGG
GGTN^NNNNNACC
E46520010
  
E466100040
BstX2 IXho II
R^GATCY
YCTAG^R
E22950030
  Not blocked by 
RG(6mA)TCY methylation  
E2302500120
BsuR IHae III
GG^CC
CC^GG
E05310005
  
E054500020
Btr IBtr I
CAC^GTC
GTG^CAG
E27710020
  
E27850080
Cci IBspH I
T^CATGA
AGTAC^T
E565100050
  
E5665000200
CciN INot I
GC^GGCCGC
CGCCGG^CG
E20320020
  Blocked by 
G(5mC)GGC(5mC)GC methylation  
E204100080
Dra IAha III
TTT^AAA
AAA^TTT
E055100010
  
E056500040
DraIIIDraIII
CACNNN^GTG
GTG^NNNCAC
E30950025
  
E3102500100
Dri IEam1105 I
GACNNN^NNGTC
CTGNN^NNNCAG
E19320010
  
E194100040
DseD IDrd I
GACNNNN^NNGTC
CTGNN^NNNNCAG
E24150080
  
E2422500320
EcoICR ISac I
GAG^CTC
CTC^GAG
E4692007
  
E470100028
EcoR IEcoR I
G^AATTC
CTTAA^G
E057500010
    
E0582500040
E057X500010
E058X2500040
EcoR VEcoR V
GAT^ATC
CTA^TAG
E059200020
  New package  
E0601000080
Ege I(Nar I)
GGC^GCC
CCG^CGG
E24320030
  
E2441000120
Erh ISty I
C^CWWGG
GGWWC^C
E06110006
  
E062500024
FaeINlaIII
CATG^
^GTAC
E4955010
  Blocked by C(6mA)TG methylation  
E49625040
FaiIFaiI
YA^TR
RT^AY
E5515080
  
E552250320
FalIFalI
^(N)8AAGNNNNNCTT(N)13^
^(N)13TTCNNNNNGAA(N)8^
E15310040
  
E154500160
FatINlaIII
^CATG
GTAC^
E15510050
  Blocked by (5mC)ATG methylation    
E156500200
Fau IFau I
CCCGC(N)4^
GGGCG(N)6^
E2095050
  Blocked by 
CC(5mC)GC methylation  
E210250200
FauND INde I
CA^TATG
GTAT^AC
E00950010
    
E010250040
Fbl IAcc I
GT^MKAC
CAKM^TG
E27110010
  
E27250040
Fok IFok I
GGATG(N)9^
CCTAC(N)13^
E2471005
  
E24850020
FriO IBan II
GRGCY^C
C^YCGRG
E157100025
  
E1585000100
Fsp4H IFnu4H I
GC^NGC
CGN^CG
E09510020
    
E09650080
Gla IGla I
G(5mC)^G(5mC)
(5mC)G^(5mC)G
E49310080
    New product  Does not cleave DNA sequence G(4mC)G(4mC)  
E494500320
Glu IGlu I
(5mC)G(5mC)^NG(5mC)G
G(5mC)GN^(5mC)G(5mC)
E51910080
    New product  
E520500320
Gsa IBseY I
CCCAG^C
G^GGTCG
E563100050
  
E5645000200
Hae IIIHae III
GG^CC
CC^GG
E067200010
  New package    
E0681000040
E068X1000040
Hga IHga I
GACGC(N)5^
CTGCG(N)10^
E4615025
  Blocked by 
GA(5mC)GC methylation  
E462250100
Hind IIHind II
GTY^RAC
CAR^YTG
E201100030
  
E2025000120
Hind IIIHind III
A^AGCTT
TTCGA^A
E073500010
    
E0742500040
E073X500010
E074X2500040
Hinf IHinf I
G^ANTC
CTNA^G
E075100010
    
E076500040
E075X100010
E076X500040
Hpa IHpa I
GTT^AAC
CAA^TTG
E07750015
  
E078250060
Hpa IIHpa II
C^CGG
GGC^C
E1615007
  Blocked by 
C(5mC)GG methylation    
E162250028
HspA I(Hha I)
G^CGC
CGC^G
E069100015
  Blocked by 
5`-G(5mC)GC-3`/3`-CG(5mC)G-5` methylation  Not blocked by  
5`-GCG(5mC)-3`/3`-CGCG-5` methylation    
E070500060
Kpn IKpn I
GGTAC^C
C^CATGG
E079200015
  Not blocked by  
GGTAC(5mC) methylation    
E0801000060
E079X200015
E080X1000060
Ksp22 IBcl I
T^GATCA
ACTAG^T
E081100010
  Blocked by 
TG(6mA)TCA methylation    
E082500040
Kzo9 IMbo I
^GATC
CTAG^
E18720025
  Blocked by 
GAT(5mC) methylation  Not blocked by 
G(6mA)TC methylation    
E1881000100
Mab ISexA I
A^CCWGGT
TGGWCC^A
E12120030
  Not blocked by 
AC(5mC)WGGT methylation  
E1221000120
Mal IDpn I
G(mA)^TC
CT^(mA)G
E489505
  
E49025020
Mbo IIMbo II
GAAGA(N)8^
CTTCT(N)7^
E47120025
  New package  Blocked by 
GAAG(6mA) methylation  
E4721000100
Mhl ISdu I
GDGCH^C
C^HCGDG
E04950035
  
E0502500140
Mlu IMlu I
A^CGCGT
TGCGC^A
E085100020
  
E086500080
Mly113 INar I
GG^CGCC
CCGC^GG
E18920030
  
E1901000120
Mnl IMnl I
CCTC(N)7^
GGAG(N)6^
E48150040
  New package  Blocked by 
CCT(5mC) methylation  
E4822500160
MroN I(Nae I)
G^CCGGC
CGGCC^G
E08750025
  
E0882500100
MroX IXmn I
GAANN^NNTTC
CTTNN^NNAAG
E24920010
    
E250100040
Msp IHpa II
C^CGG
GGC^C
E091100010
    
E092500040
Msp20 IBal I
TGG^CCA
ACC^GGT
E30110020
    
E30250080
MspA1 INspB II
CMG^CKG
GKC^GMC
E19150025
  
E1922500100
MspR9 IScrF I
CC^NGG
GGN^CC
E17550025
  Blocked by 
C(5mC)NGG methylation  
E1762500100
Nru INru I
TCG^CGA
AGC^GCT
E09950025
  Blocked by 
TCGCG(6mA) methylation  
E1002500100
PalA IAsc I
GG^CGCGCC
CCGCGC^GG
E48310015
  Blocked by 
GG(5mC)G(5mC)GCC methylation  
E48450060
Pce IStu I
AGG^CCT
TCC^GGA
E105100015
  
E106500060
Pci IBspLU11 I
A^CATGT
TGTAC^A
E27520045
  Blocked by (6mA)CATGT methylation.  Not blocked by 
AC(6mA)TGT methylation.    
E2761000180
PciS ISap I
GCTCTTC(N)1^
CGAGAAG(N)4^
E4975050
  
E498250200
Pct IBsm I
GAATGCN^
CTTAC^GN
E045100050
    
E0465000200
Ple19 IPvu I
CGAT^CG
GC^TAGC
E19510020
  Not blocked by 
CG(6mA)TCG methylation  
E19650080
Pps IPle I
GAGTC(N)4^
CTCAG(N)5^
E2692540
  
E270125160
Psi IPsi I
TTA^TAA
AAT^ATT
E27910070
    
E280500280
Psp124B ISac I
GAGCT^C
C^TCGAG
E107100020
  
E108500080
Psp6 IEcoR II
^CCWGG
GGWCC^
E45310020
  Blocked by 
C(5mC)WGG methylation  
E45450080
PspC IPmaC I
CAC^GTG
GTG^CAC
E475200015
  
E4761000060
PspE IBstE II
G^GTNACC
CCANTG^G
E169200030
  
E17010000120
PspL ISpl I
C^GTACG
GCATG^C
E22330040
  
E2241500160
PspN4 INla IV
GGN^NCC
CCN^NGG
E089100050
  Blocked by 
5`-GGNN(5mC)C-3`/3-C(5mC)NNGG-5` or 
5`-GGNN(5mC)C-3`/3-CCNNGG-5`
methylation   
E0905000200
PspOM IApa I ()
G^GGCCC
CCCGG^G
E215100020
  
E216500080
PspPP IPpuM I
RG^GWCCY
YCCWG^GR
E25510010
  Blocked by 
RGGWC(5mC)Y methylation  
E25650040
PspX IPspX I
VC^TCGAGB
BGAGCT^CV
E47720060
    
E4781000240
E478X1000240
Psr IPsr I
^(N)7GAACNNNNNNTAC(N)12^
^(N)12CTTGNNNNNNATG(N)7^
E13110050
  
E132500200
Pst IPst I
CTGCA^G
G^ACGTC
E109400015
  
E1102000060
E109X400015
E110X2000060
Pvu IIPvu II
CAG^CTG
GTC^GAC
E111200015
    
E1121000060
Rga ISgf I
GCGAT^CGC
CGC^TAGCG
E49120015
  Not blocked by 
GCG(6mA)TCGC methylation  
E492100060
Rig IFse I
GGCCGG^CC
CC^GGCCGG
E52910060
  
E530500240
Rsa IRsa I
GT^AC
CA^TG
E113100030
    
E1145000120
RsaN IRsa I
G^TAC
CAT^G
E55520040
  
E5561000160
Rsr2 IRsr II
CG^GWCCG
GCCWG^GC
E2811000120
  
E2825000480
Sal ISal I
G^TCGAC
CAGCT^G
E115200030
  
E11610000120
Sbf ISse8387 I
CCTGCA^GG
GG^ACGTCC
E10120030
  
E1021000120
Set ISet I
ASST^
^TSSA
E53720050
  New product  
E5381000200
E538X1000200
SfaN ISfaN I
GCATC(N)5^
CGTAG(N)9^
E16550060
    
E1662500240
Sfi ISfi I
GGCCNNNN^NGGCC
CCGGN^NNNNCCGG
E123100025
  Blocked by 
 GGC(5mC)NNNNNGGCC methylation  Not blocked by 
GGCCNNNNNGGC(5mC) methylation  
E1245000100
E123X100025
E124X5000100
Sfr274 IXho I
C^TCGAG
GAGCT^C
E125200015
  Blocked by 
 CTCG(6mA)G methylation  Not blocked by 
CT(5mC)GAG methylation  
E1261000060
Sfr303 ISac II
CCGC^GG
GG^CGCC
E127100015
  
E128500060
Sma ISma I
CCC^GGG
GGG^CCC
E177200030
  
E17810000120
Smi ISwa I
ATTT^AAAT
TAAA^TTTA
E225100025
  
E2265000100
SmiM IMsl I
CAYNN^NNRTG
GTRNN^NNYAC
E29320025
  
E2941000100
Sph ISph I
GCATG^C
C^GTACG
E12950040
  New package  
E1302500160
Sse9 ITsp509 I
^AATT
TTAA^
E21750020
  New package    
E218250080
Ssp ISsp I
AAT^ATT
TTA^TAA
E04150025
  Blocked by  
A(6mA)TATT methylation.  
E0422500100
Taq ITaq I
T^CGA
AGC^T
E13320005
  New package    
E1341000020
Tru9 IMse I
T^TAA
AAT^T
E19950040
  New package  Blocked by TTA(6mA) methylation    
E2002500160
Tth111 ITth111 I
GACN^NNGTC
CTGNN^NCAG
E09740020
  
E098200080
Vne IApaL I
G^TGCAC
CACGT^G
E137100020
  
E138500080
Vsp IVsp I
AT^TAAT
TAAT^TA
E139100020
  Blocked by ATTA(6mA)T methylation     
E140500080
Xba IXba I
T^CTAGA
AGATC^T
E141200015
  New package  Blocked by 
TCTAG(6mA) methylation    
E1421000060
E141X200015
E142X1000060
Xma IXma I
C^CCGGG
GGGCC^C
E23330040
  New package  
E2341500160
ZraI(AatII)
GAC^GTC
CTG^CAG
E46320075
  
E4641000300
Zrm ISca I
AGT^ACT
TCA^TGA
E00560015
  
E006300060
Zsp2 IAva III
ATGCA^T
T^ACGTA
E145100030
  
E1465000120


 
Degenerate nucleotides
R = A or G; W = A or T; S = G or C; V = A or C or G;
K = G or T; M = A or C; Y = T or C; B = C or G or T;
D = A or G or T;H = A or C or T; N = A or C or G or T;

 
The icons description
New productNew product package
You can read the scientific paper about this product. Click on the icon to read the paper.
Product is qualified for mammalian genome studies. Click on the icon to read the paper.
Enzyme is blocked by presence of corresponding methylated base within the recognition sequence (see pop-up information or click on the enzyme name to read the detailed description)
Enzyme is NOT blocked by presence of corresponding methylated base within the recognition sequence (see pop-up information or click on the enzyme name to read the detailed description)

 
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